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American Society of Microbiology ABMM Sample Questions:

1. A public health investigation is underway following several cases of encephalitis in a specific geographic region during the summer months. Mosquitoes are abundant in the area. Serological testing of patient CSF samples reveals IgM antibodies against a flavivirus. The MOST likely causative agent is:

A) Jamestown Canyon virus
B) Eastern equine encephalitis virus
C) West Nile virus
D) Rabies virus

2. Which biosafety level (BSL) is required for clinical laboratory work involving the propagation (culture) of Coccidioides immitis/posadasii?

A) BSL-1
B) BSL-2
C) BSL-4
D) BSL-3

3. A patient presents with symptoms suggestive of primary syphilis. Which diagnostic test typically becomes positive earliest in the course of infection?

A) Treponema pallidum particle agglutination (TP-PA)
B) Venereal Disease Research Laboratory (VDRL) test on CSF
C) Darkfield microscopy of lesion exudate
D) Rapid Plasma Reagin (RPR)

4. During a primary infection with Epstein-Barr virus (EBV), the virus establishes latency in B lymphocytes.
The transition from lytic to latent infection is tightly regulated by viral gene expression. Which of the following viral proteins is crucial for the establishment and maintenance of EBV latency and is a key target in EBV-associated lymphomas?

A) Epstein-Barr nuclear antigen 1 (EBNA1)
B) Viral capsid antigen (VCA)
C) Glycoprotein gp350
D) Early antigen (EA)

5. A clinical microbiology laboratory is preparing to validate a new molecular assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA). To assess the positive predictive value (PPV) of the assay, they need to know:

A) The number of true positive results divided by the total number of true positive and false negative results.
B) The number of true negative results divided by the total number of negative test results.
C) The number of true negative results divided by the total number of true negative and false positive results.
D) The number of true positive results divided by the total number of positive test results.

Solutions: